植物染色体G-带的初步研究

本文首次报道了川百台(Lilium davidii)、华山松(Pinus armardii)和七叶一枝花(Paris polyphylla)等植物染色体G-带研究结果。本试验的G-带与以往的C-带不同,C-带每条染色体上一般只有1-4条带,多分布在着丝点附近,而G-带则多达几十条,分布在整条染色体上,带纹清晰,前期染色体带呈颗粒状,中期染色体呈明显的带状,与哺乳动物染色体G-带很相似。G-带的数目取决于染色体浓缩的程度。前期染色体带纹数目是中期的三倍,接近人类高分辨带水平。对G-带带纹采用了自动光谱分析,波峰数值与带纹相符。作者同时介绍了胰酶法在植物染色体G-带中的应用。认为此方法既适合动物亦适用于植物。但植物G-带显示的关键可能不在胰酶法本身,而在合适的分裂时期及染色体处理技术。

A PRELIMINARY STUDY ON THE G-BANDS OF CHROMOSONES IN SOME PLANTS

In this joint paper the authors first report on the results of the G-bands of chromosomes of Lilium davidii, Pinus armardii and Paris polyphylla. By our observation, G-bands differ from the C-bands observed before. Usually, the chromosomes of these plants mentioned above display 1-4 C-bands, and they are distributed usually in the centromere regions; the G-bands appear as many as tens. All of them are distributed in the entire length of the chromosomes, and are clear enough. G-bands display granular state in the prophase figure, and display the clear bands in the metaphase. These are quite similar to the G-bands in mammalian chromosomes. G-bands number depends on the degree of chromosomal contraction. Band number of prometaphase chromosomes is three times as many as that of metaphase chromosomes, and is near to the human High-Resolution bands level. G-bands were analysed by the automatic spectrum analysis, and the wave crest value conformed with the Gbands. In this paper, the authors introduce the application of trypsin method in G-bands of plant chromosomes. It indicates that this method is not only suitable to animals, but also to plants. However, the key points of G-bands for plant chromosomes seem to be the suitable division phase and the treatment technique of chromosomes rather than the trypsin method itself.