小麦CEN/TFL1-like cDNA克隆及其编码序列分析

以水稻、金鱼草、拟南芥CEN/TFL1蛋白质序列为参考,以小麦中CEN/TFL1基因同源EST片段为依据设计特异引物,利用RT-PCR从普通小麦‘中国春’幼穗总RNA中扩增出549 bp的特异片段。测序结果表明,该片段包含了完整的ORF,编码产物为典型的CEN/TFL1-like蛋白家族成员。序列比对表明,该基因编码产物与黑麦草LpTFL1、水稻FDR2和FDR1以及玉米ZmTFL1亲缘关系最近,分别具有96.5%、96.0%、93.6%和95.4%的相似性;与哺乳动物Rattus norveqicus磷脂酰乙醇胺结合蛋白(PEBP)具有38.9%相似性。空间结构预测表明,该蛋白具有PEBP家族成员的典型三维结构。

Cloning and Amino Acid Sequence Analysis of CEN/TFL1-like Gene in Common Wheat

Using specific primers based on common wheat cultivar Chinese spring ESTs,which are homologous to CEN,TFL1 in Antirrhinum and Arabidopsis respectively,we obtained a fragment of wheat CEN/TFL1 like gene from total RNA of young inflorescence of 'Chinese Spring' by RT-PCR.Amino acid sequence alignment showed that CEN/TFL1-like had 96.5%,96%,93.6% and 95.4% similarity with LpTFL1,FDR2,RDR1 and ZmTFL1 respectively;It also had 38.9% similarity with Phosphatidylethanolamine-binding protein in mammal Rattus norveqicus.Protein structure prediction results showed that it has a highly conserved domain of PEBP family.