Carbohydrate composition of normal and variant human alpha 1-protease inhibitors. |
| |
Authors: | A Yoshida T Mega |
| |
Institution: | Department of Biochemical Genetics, City of Hope Medical Center, Duarte, California 91010 U.S.A. |
| |
Abstract: | Carbohydrate composition of normal human alpha 1-protease inhibitor (PiM1) and several variant inhibitors (PiM2, PiM3, PiA, PiS, and PiZ) was determined by methanolysis of the samples followed by quantitative analysis of both neutral and amino sugars using gas-liquid chromatography. All normal and variant inhibitors contained nine mannose, seven galactose, ten N-acetylglucosamine, and eight N-acetylneuraminic acid residues per molecule, and no significant difference was found in their carbohydrate compositions. PiA is a variant with the fastest anodal electrophoretic mobility, and PiZ is a variant with the slowest mobility thus far reported. The differences in electrophoretic mobility of these Pi variants are entirely due to their amino acid substitutions determined previously. These amino acid substitutions have no effect on the carbohydrate structure of the protease inhibitor. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|