| 植物乳杆菌CLP0279发酵生产低温SOD培养基的优化 |
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| 引用本文： | 李晓艳,于爽,窦少华,张庆芳,迟乃玉,王晓辉.植物乳杆菌CLP0279发酵生产低温SOD培养基的优化[J].微生物学通报,2016,43(10):2129-2137. |
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| 作者姓名： | 李晓艳 于爽 窦少华 张庆芳 迟乃玉 王晓辉 |
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| 作者单位： | 大连大学生命科学与技术学院 辽宁 大连 116622,大连大学生命科学与技术学院 辽宁 大连 116622,大连大学生命科学与技术学院 辽宁 大连 116622,大连大学生命科学与技术学院 辽宁 大连 116622,大连大学生命科学与技术学院 辽宁 大连 116622,大连大学生命科学与技术学院 辽宁 大连 116622 |
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| 基金项目： | 国家高技术研究发展计划项目(863计划) (No. 2014AA093512)；国家自然科学基金项目(No. 31500039) |
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| 摘 要： | 【目的】提高植物乳杆菌CLP0279发酵生产低温超氧化物歧化酶(superoxide dismutase，SOD)的能力。【方法】在单因素实验基础上，采用Plackett-Burman (PB)设计、Box-Behnken (BB)设计和响应面分析法(RSM)，对发酵培养基进行优化。【结果】植物乳杆菌CLP0279产低温SOD最佳发酵培养基(g/L)：玉米粉25.000，磷酸二氢钾2.600，磷酸氢二钾1.830，硫酸铜0.011，硫酸锌0.014。在最佳培养基条件下产酶活力达到194.82 U/ml，是优化前的1.36倍。【结论】通过响应面分析，对植物乳杆菌CLP0279发酵生产低温SOD的培养基进行优化，明显提高了产酶能力。确定了磷酸氢二钾、硫酸铜和硫酸铵为发酵培养基中影响酶活的3个关键因子。研究结果为SOD的发酵放大提供了依据。
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| 关 键 词： | 植物乳杆菌，低温超氧化物歧化酶，发酵培养基，优化 |
Optimization of fermentation medium for a cold-adapted superoxide dismutase (SOD) produced by Lactobacillus plantarum sp. CLP0279 |
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| LI Xiao-Yan,YU Shuang,DOU Shao-Hu,ZHANG Qing-Fang,CHI Nai-Yu and WANG Xiao-Hui.Optimization of fermentation medium for a cold-adapted superoxide dismutase (SOD) produced by Lactobacillus plantarum sp. CLP0279[J].Microbiology,2016,43(10):2129-2137. |
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| Authors: | LI Xiao-Yan YU Shuang DOU Shao-Hu ZHANG Qing-Fang CHI Nai-Yu and WANG Xiao-Hui |
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| Institution: | College of life science and Technology, Dalian University, Dalian, Liaoning 116622, China,College of life science and Technology, Dalian University, Dalian, Liaoning 116622, China,College of life science and Technology, Dalian University, Dalian, Liaoning 116622, China,College of life science and Technology, Dalian University, Dalian, Liaoning 116622, China,College of life science and Technology, Dalian University, Dalian, Liaoning 116622, China and College of life science and Technology, Dalian University, Dalian, Liaoning 116622, China |
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| Abstract: | Objective] In order to increase the activity of a cold-adapted superoxide dismutase (SOD) from Lactobacillus plantarum sp. CLP0279. Methods] Based on the single-factor experiment, the fermentation medium for the production of SOD was optimized using Plackett-Burman method, Box-Behnken design and response surface analysis. Results] The optimal fermentation medium (g/L) was determined as follows: corn meal powder 25.000, KH2PO4 2.600, K2HPO4 1.830, CuSO4·5H2O 0.011, ZnSO4·7H2O 0.014. Under these conditions, the maximum activity of SOD was 194.82 U/mL, which was 1.36 times comparing to that before optimization. Conclusion] The activity of the cold-adapted SOD was obviously increased after optimization and three crucial factors (K2HPO4, CuSO4·5H2O and ZnSO4·7H2O) were verified by the response surface analysis, which might provide a foundation for the scale-up culture in the future. |
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| Keywords: | Lactobacillus plantarum Cold-adapted SOD Fermentation medium Optimization |
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