钙调素在IAA和6-BA诱导绿豆下胚轴原生质体膨大过程中的含量变化

含钙培养液和含激素培养液中的绿豆下胚轴原生质体在培养30min时分别检测到钙调素（CaM）峰，在含钙培养液中加入IAA或6-8ACaM含量急剧降低，这与先前的试验结果即45Ca2 积累增多和体积膨大正好对应。异博定（verapamil）、LaCl3、EGTA或W7可使激素 CaCl2处理的CaM含量不同程度地回升，甚至接近或超过对照的水平。而A23187处理或K 、Zn2 等代替Ca2 则使CaM含量保持在与激素处理相似的低水平。这也与先前观察到的45Ca2 积累和体积的变化相对应。表明钙调素在IAA和6-8A诱导绿豆下胚轴原生质体膨大过程中的境与Ca2 共同起着调节作用。

CHANGES IN CONTENT OF CALMODULIN IN IAA-AND 6-BA-INDUCED SWELLING OF PROTOPLASTS FROM HYPOCOTYL IN ETIOLATED MUNG BEAN SEEDLINGS

Calmodulin (CaM) content in protoplasts cultured with CaCl2-or IAA (or 6-BA)-bearing medium raised after being incubated for 30 minutes. When treated with IAA (or68A) CaCl2, the CaM content decreased sharply, which just coincided with the increase of45Ca2 accumulation and protoplast swelling as indicated in our previous studies. Eitherverapadril, LaCl3, EGTA or W7 could increase the low CaM content to an approximate oreven higher value than that of control. A23187 and cations such as K and Zn2 ,however, could reduce CaM level just as the influences of IAA or 6-BA with Ca2 . It wasalso consistent with the changes of 45Ca2 accumulation and protoplast volume. Theseresults showed that CaM did act with Ca2 during the modulation of protoplast swellinginduced by IAA or 6-BA.