Dimers of nicotinamide adenine dinucleotide: New evidence for the structure and the involvement in an enzymatic redox process |
| |
Authors: | Vincenzo Carelli Felice Liberatore Antonio Casini Rosanna Mondelli Alberto Arnone Italo Carelli Giuseppe Rotilio Irene Mavelli |
| |
Institution: | 1. Cattedra di Chimica Farmaceutica Applicata, Università di Roma, Roma, Italy;2. Centro di Studio per le Sostanze Organiche Naturali del CNR, Milano, Italy;3. Centro di Studio per l''Elettrochimica e la Chimica Fisica delle Interfasi del CNR, Roma, Italy;4. Centro di Biologia Molecolare del CNR, Roma, Italy |
| |
Abstract: | The composition and the structure of the product from the known electrochemical dimerization of the NAD+ have been conclusively demonstrated. A detailed analysis of the 1H and 13C nmr spectra has in fact led to the conclusion that the product contains three diastereoisomeric dimers of the 4,4′-tetrahydrobipyridyl type. Furthermore, the cytoplasmic fraction obtained from a standard mitochondrial preparation of rat liver has been shown to catalyze the oxygen uptake by the dimers. A 1 : 1 molar ratio of the reagents in the redox process is indicated by manometric data on oxygen uptake complemented by spectrophotometric analysis of the oxidized substrates, suggesting that H2O2 is the reduction product. NAD+ was identified as the oxidation product by an enzymatic method. |
| |
Keywords: | To whom reprint requests should be sent at: Cattedra di Chimica Farmaceutica Applicata Università di Roma Piazzale Aldo Moro 7 00100 Roma Italy |
本文献已被 ScienceDirect 等数据库收录! |