埃斯基红豆草下胚轴愈伤组织原生质体的培养与植株再生

埃斯基红豆幼苗的下胚轴切段在附加２，４－Ｄ０．５ｍｇ／Ｌ，ＫＴ１ｍｇ／Ｌ的ＭＳ中形成胚性愈伤组织。来自１１－１３个月龄、继代６－１５天的愈伤组织的原生质体，在改良的Ｖ－ＫＭ液体培养基中可持续分裂形成细胞团，培养１０天时的分裂率和克隆率分别为６５．８８％和５３．３８％周后就可将将原生质体形成的小愈伤组织转于培养基上。原生质体在改良的Ｂ５液体培养基也可以分裂形成小愈伤组织，但分裂率低于Ｖ－ＫＭ。来自原

CULTURE AND PLANT REGENERAION OF PROTOPLASTS FROM HYPOCOTYL CALLI OF SAINFOIN(Onobrychis viciae folia Scop.)CULTIVAR ESKI

Embryogenic calli was able to be formed from sainfoin cv. Eski hypocotyl explants culturedon MS medium containing 2,4-D0.5mg/L,KT1mg/L. Protoplasts were isolated from the callicultured continuously for 11-13 months after being transferred 6-15 days. When cultured inmodified liquid V-KM,the protoplast sustained division and formed cell colonies. At 10 days,thefrequency of cell division and colony formation are 65.88% and 53.38% respectively. Microcallicould be transferred in semisolid media after 5 weeks. The protoplasts were also to divide andforme microcalli in the modified B_5 medium,but the division freqtiency was lower than in V-KMmedium. Protoplasts-derived microcalli exhibited active growth and regenerated roots and buds whentransferred onto solid (0.5%agrose)media. Shoots were obtained from the calli on MS mediumplus BA 0.5-1mg/L and NAA 0.1-0.5mg/L. Roots were induced from the shcots on 1/2MS medium plus BA 0.02mg/L and NAA 0.2mg/L.