| 人U_1和U_2snRNA基因定位缺失和取代突变 |
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| 引用本文： | 韩玉珉.人U_1和U_2snRNA基因定位缺失和取代突变[J].中国生物化学与分子生物学报,1990,6(1):66-70. |
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| 作者姓名： | 韩玉珉 |
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| 作者单位： | 中国科学院生物物理研究所 北京 |
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| 摘 要： | 用寡聚核苷酸诱导的定位突变法,将人U_1和U_2snRNA基因的5'-端调控区域的一段能与SV_(40)T抗原相结合的DNA删去,造成缺失突变,改变这段DNA核苷酸的排列顺序,造成取代突变。突变株用原位杂交法筛选,由限制性内切酶电泳图谱分析和DNA顺序测定得到证实。突变率约为5％。
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| 关 键 词： | U_1U_2基因 定位突变 缺失 取代 |
| 收稿时间： | 1990-02-20 |
The Specific Deletion and Substitution Mutagenesis of Human U1 and U2 snRNA Gene |
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| Han Yu-min.The Specific Deletion and Substitution Mutagenesis of Human U1 and U2 snRNA Gene[J].Chinese Journal of Biochemistry and Molecular Biology,1990,6(1):66-70. |
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| Authors: | Han Yu-min |
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| Institution: | (Institute of Biophysics, Academia Sinica, Beijing |
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| Abstract: | Using the oligonucleotide directed mutagesis we have deleted a segment from DNA region 5'-flanking of human U1. and U2 snRNA gene which can be bound by SV40 T-antigen. We have also substituted this DNA region with changed nucleotide sequences. The mutants had been screened by in situ colony hybridization. The mutants selected had been characterized by restriction enzyme mapping and DNA sequening. The efficiency of mutagenesis was about 5%. |
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| Keywords: | U1 U2 gene Mutagenesis Deletion Substitution |
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