植物组织石蜡切片的扫描电镜观察方法研究

石蜡切片的扫描电镜观察法有其独到之处:集光镜和扫捕电镜特长于一体,在大量的石蜡切片光镜观察的基础上,挑选具有研究线索的切片,采用此法转移到扫描电镜下作高分辩研究,既可普查切片全貌,又可处得切片中亚微结构的三维图像,这对结构的准确分辩十分有利,且便于作连续切片观察。本文简要介绍这一实验技术。

A TECHNIQUE OF OBSERVATION ON PARAFFIN SECTIONS WITH SCANNING ELECTRON MICROSCOPE

The technique for observation on the paraffin sections with SEM is useful for biological researches. The images obtained by this method can show distinet three dimensional structure on the sections. Formerly there were two principal problems with this technique: First, the remanent paraffin in sections influenced the contrast of the images. Second, the missing and place movement of the struetures in the sections occurred because of the buoyancy of the liquid (xylene)in the course of dewaxing. We have solved the two problems in two ways: First, before dewaxing, cover the sections closely with a thin formvar film with many pores to avoid the missing and place movement of the structures. Secondly, dewax the section in a sealed circulative device to raise the efficiency of dewaxing and clear away the remanent paraffin in the sections completely. As a result, very distinct images can be obtained even in high magnificatious.