Cloning and characterization of restriction fragments of phage Mu DNA |
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Authors: | Matthias Staufenbiel Wolfgang Schumann |
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Institution: | Fakultät für Biologie, Universität Konstanz, D-7750 Konstanz, F.R.G. |
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Abstract: | Abstract The Eco RI-A and B, the Bam HI-C and the two Eco RI- Bam HI restriction fragments of transposing phage Mu DNA were inserted into vector plasmids pRSF2124 and pBR322. Quantitative marker rescue experiments for five genes located on the Eco RI-A fragment revealed complementation of phages carrying amber mutations in genes C , E , H , F and L . The in vitro coding capacity of the recombinant plasmids was assayed in a DNA-directed protein synthesizing system. |
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Keywords: | Phage Mu recombinant plasmids marker rescue complementation DNA-directed protein synthesis coding capacity |
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