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In vivo detection of intrinsic reactive oxygen species using acyl-protected hydroxylamine in puromycin nephrosis |
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| Authors: | Ueda Atsushi Hirayama Aki Nagase Sohji Inoue Mariko Oteki Takaaki Aoyama Masaaki Yokoyama Hidekatsu |
| Affiliation: | a Department of Internal Medicine, Namegata District General Hospital, Ibaraki, Japanb Pathophysiology of Renal Diseases, Medical Sciences for Control of Pathological Processes, Graduate School of Comprehensive Human Science, University of Tsukuba, Ibaraki, Japanc Center for Clinical Medicine and Research, International University of Health and Welfare, Tochigi, Japand Yamagata Promotional Organization for Industrial Technology, Yamagata, Japane Nanolabeling and Measurement Research Group, National Institute of Advance Industrial Science and Technology, Nagoya, Japan |
| Abstract: | Intrinsic reactive oxygen species (ROS) in a rat model of human minimal change nephropathy were detected directly using an in vivo electron paramagnetic resonance (EPR) method with 1-acetoxy-3-carbamoyl-2,2,5,5-tetramethylpyrrolidine (ACP) in real time. The nephrosis was induced by the intravenous administration of 75 mg/kg of puromycin aminonucleoside (PAN). It was found that ROS in the kidney were increased 1 h after the administration of PAN. This increased oxidative stress declined at 24 h and returned to a normal level 3 days after PAN administration. This is the first non-invasive in vivo detection and quantification of specific ROS in an experimental nephrosis model. |
| Keywords: | Reactive oxygen species acyl-protected hydroxylamine puromycin aminonucleoside nephrosis electron paramagnetic resonance |
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