虎杖甙对家兔肺缺血／再灌注损伤肺组织TLR4和ICAM-1表达的影响

目的：研究家兔肺缺血／再灌注损伤时Toll样受体4（TLR4）信号转导通路变化及虎杖甙（PD）对其影响。方法：复制在体肺缺血／再灌注损伤模型。健康日本大耳白免30只,随机分为对照（C）组、缺血／再灌注（I／R）组、PD组。鲎试剂试管法检测血浆内毒素（ET）;RT-PCR法检测肺组织TLR4、核因子-KBp65（NF-KBp65）和细胞间粘附分子-1（ICAM-1）mRNA表达;苏木素-伊红（HE）染色,光镜观察肺组织形态学变化。结果：I／R组、PD组与C组相比血浆ET无显著差异（均P〉0．05）。I／R组肺组织TLR4、NF-KBp65及ICAM—1mRNA表达较C组显著升高（均P〈0．01）;PD组上述改变较I／R组明显下降,但仍高于C组（均P〈0．01）;光镜见PD组肺组织结构损伤明显轻于I／R组。结论：PD治疗可以下调肺组织TLR4、NF-kBp65表达,进而抑制ICAM-1等炎症介质的转录和分泌,减轻肺缺血／再灌注损伤。

The regulative effects of polydatin on toll-like receptor 4 signal transduction pathway in lung ischemia/reperfusion injury in rabbits

Aim： To investigate protective effects of polydatin（PD） during lung ischemia/reperfusion in rabbits and its potential inechanisms. Me thods： Rabbit lung modal of ischemia/reperfusion（I/R） injury was constituted in vivo. Thirty rabbits were divided into groups randomly： Control（ C）, I/R, PD group, respectively. Endotoxin（ET） in plasma was analyzed by End-point Chromogenic Assay, the expression of Toll-like receptor 4（TLR4） mRNA, nuclear factor（NF）-KBp65 mRNA, intracellular adhesion molecule-1 （ICAM-1） mRNA were measured by RT-PCR, the morphological changes of lung tissue were observed with hematoxylin-eosin （HE） staining. Results： There was no significant difference in ET concentration of plasma between groups （all of P 〉 0.05）. The expression of TLR-4 mRNA, NF-KBp65 mRNA and ICAM-1mRNA in I/ R group were significantly increased as compared to C group and PD group, while those expressions in PD group were evidently higher than those in C group（all of P 〈 0.01）. Light microscope sbow＇ed that the lung pathological injuries in PD group were obviously alleviated as compared to I/R group. Conclusion： PD might have a protective effect on lung ischemia/reperfusion injury by down-regulating TLR4 and NF-KB expression, then inhibiting the release of mediators of inflan,nation as ICAM- 1.