尼尔雌醇对去卵巢大鼠骨元素代谢和相关激素含量的影响

目的：测定犬鼠骨矿物元素含量和血清相关激素及IL-6含量,研究尼尔雌醇（CCE3）对去卵巢（OVX）大鼠骨元素代谢和相关激素含量的影响。方法：健康4月龄雌性SD大鼠24只随机分成4组（n=6）：正常对照组;假手术组;去卵巢组;去卵巢＋CCE3组。去卵巢＋CCE3组大鼠于去卵巢手术后第2天开始给予CCE3灌胃,1ms／ks体重,每周1次,持续11周。结果：去卵巢组大鼠骨Ca、Mg、S、Co、Mn、Zn等元素含量显著降低,骨P（磷）含量升高,血清岛、P（孕酮）、TSH、T4、CT、Cortisol、GH等含量显著降低,IL-6、FSH、LH等含量显著升高。CCE3可使去卵巢大鼠骨Ca、Mg、Co、Mn、S、Zn等含量回升,骨P（磷）含量回降,血清E2、P（孕酮）、TSH、T4、CT、Cortisol、GH等显著回升,IL-6、FSH、LH等含量显著回降。结论：CCE3可纠正去卵巢所致的骨元素代谢紊乱,CCE3改变去卵巢大鼠体内相关激素和IL-6水平是CCE3去卵巢大鼠骨元素代谢紊乱的重要机制。

Mechanism of the influence of CCE3 on bone mineral element metabolism in ovariectomized rats

Aim： To investigate the influence of CCE3 on bone mineral element metabolism and relative hormone in ovariectomized rats. Methods： 24 female SD rats were divided randomly into 4 groups（ n = 6）： （1）Normal control; （2）sham ovariectomy; （3）ovariectomy（OVX） ; （4）OVX ＋ CCE3. Results： （1）Ca, Mg, S, Co, Mn, Zn reduced obviously and P increased in the bone of the OVX rats. （2）E2, P, TSH, T4, CF, Cortisol, GH reduced obviously and IL-6, FSH, LH increased obviously in serum of the OVX rats. （3）CCE3 corrected the changes of Ca, Mg, S, Co, Mn, Zn and P in bone as well as the changes of E2, P, TSH, T4, CT, Cortisol, GH and IL-6, FSH, LH in serum of the OVX rats. Conclusion： Changes of ralative hormone and IL-6 induced by CCE3 are the important mechanism of the influence of CCE3 on bone mineral element metabolism in ovafiectomized rats.