Abstract: | The kinetics of 14C-2-acetate assimilation by Chlorella pyrenoidosain the light were examined. Under aerobic conditions the primaryproduct of acetate assimilation was succinic acid which, afterten seconds, contained over 60 per cent of the 14C incorporatedby the cells. The percentage of the total 14C in succinate fellwith time, while that in citrate and glutamate increased. After1800 sec over 60 per cent of 14C was present in two compounds,glutamic acid and an unknown compound (X). Glucose-6-phosphate,fructose-6-phosphate, phosphoglyceric acid and phosphoenolpyruvicacid became labelled after 60 sec but together never containedmore than one per cent of the total 14C incorporated. Underanaerobic conditions succinate was still the primary productof acetate assimilation, and the absence of carbon dioxide resultedin a decrease in 14C incorporation into compound X. The patternof acetate assimilation in acetate grown and acetate adaptedChlorella was very similar to that in photo-autotrophicallygrown Chlorella. In the presence of 106M DCMU, succinicacid was the primary product of acetate assimilation, but therewas an early Incorporation of 14C into glutamate, aspartate,and malate. 4 x103M MFA did not effect the early incorporationof 14C into succinic acid, but resulted in accumulation of 14Cin citrate and a decreased amount in glutamate and in compound X. |