Tryptophan 7-Halogenase from Pseudomonas aureofaciens ACN Strain: Gene Cloning and Sequencing and the Enzyme Expression |
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Authors: | V N Burd K-H van Pee |
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Institution: | (1) Kupala State University, per, Dovatora 3/1, Grodno, 230012, Belarus;(2) Technical University, Dresden, Germany |
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Abstract: | The gene of tryptophan 7-halogenase was isolated from the Pseudomonas aureofaciens ACN strain producing pyrrolnitrin, a chlorocontaining antibiotic, and sequenced. A high homology degree (over 95%) was established for the genes and the corresponding halogenases from P. aureofaciens ACN and P. fluorescens BL915. The tryptophan 7-halogenase gene was amplified by PCR, and the corresponding enzyme was expressed in Escherichia coli cells using the pBSII SK+ vector. |
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Keywords: | halogenase Pseudomonas pyrrolnitrin tryptophan |
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