Differentiation of Yam Virus Isolates Using Symptomatology, Western Blot Assay, and Monoclonal Antibodies |
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| Authors: | C Goudou-Urbino L Givord J B Quiot M Boeglin G Konate J Dubern |
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| Institution: | LPRC ORSTOM BP 5035, 34032 Montpelier cedex, France;Laboratoire de Virologie, INERA KamboinséBP 476 Ouagadougou 01, Burkina Faso;CNRS Institut de Biologie Moléculaire et Cellulaire 15 rue Descartes 67084 Strasbourg, France;INRA, Laboratoire de botanique et de pathologie végétale, 9 place Viala, 34000 Montpellier cedex 01, France |
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| Abstract: | Monoclonal antibodies (mAbs) were prepared against a yam mosaic virus (YMV) isolate from the Côte d'Ivoire. Symptomatology, Western immunoblotting, and ELISA were used to discriminate 69 isolates of YMV originating from different Dioscorea species and from various yam producing areas. These isolates induced two types of symptoms, were of four different electrophoretic mobilities and formed two serogroups. These results suggest that at least six differetit groups of isolates exist, three of which infect the main cultivated species in various geographical areas while two others were from unusual samples in our collection. An isolate from 'Pilimpikou Yam' from Central Burkina Faso was serologically distinct. It is concluded that there is a significant variability among yam virus isolates which is unrelated to the origin of the isolate (geographic or host species). It is suggested that precautions should be taken in order to avoid international exchange of infected material. |
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