Genetic characterization of Paspalum notatum accessions by AFLP markers |
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Authors: | F Espinoza L D Daurelio S C Pessino E M Valle C L Quarin |
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Institution: | (1) Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, 2000 Rosario, Argentina;(2) Instituto de Botánica del Nordeste, Facultad de Ciencias Agrarias, Universidad Nacional del Nordeste, Sargento Cabral 2131, 3400 Corrientes, Argentina |
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Abstract: | Paspalum notatum Flügge is a warm-season forage grass with sexual diploid and apomictic tetraploid races. Genetic improvement was achieved
in out-breeding diploids. The acquisition of artificial sexual tetraploids has raised the possibility of performing crosses
and plant improvement at the tetraploid level. The objective of our study was to obtain a genetic and cytoembryological characterization
of a germplasm collection of P. notatum, including 31 accessions from seven countries of America and 11 experimentally obtained genotypes. Morphology of mature gametophytes
was observed to assess the mode of reproduction of the accessions. A total of 1342 AFLP fragments were generated across the
42 genotypes and from two reference taxa: P. urvillei and P. procurrens. AFLP data were converted into a binary matrix and similarity relationships were established. The genetic distance among
all the accessions showed a maximum value of 0.36. In addition, eleven AFLP fragments were observed exclusively in apomictic
plants, which could be linked to genomic regions implicated in the control of apospory. |
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Keywords: | AFLP analysis apomixis genetic diversity Paspalum notatum reproductive behaviour |
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