Genetic complementation of a floral homeotic mutation,apetala3, with an Arabidopsis thaliana gene homologous to DEFICIENS of Antirrhinum majus |
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| Authors: | Hiroyuki Okamoto Azusa Yano Hideaki Shiraishi Kiyotaka Okada Yoshiro Shimura |
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| Affiliation: | (1) Division I of Gene Expression and Regulation, National Institute for Basic Biology, 444 Okazaki, Japan;(2) Department of Biophysics, Faculty of Science, Kyoto University, 606 Kyoto, Japan;(3) Present address: Department of Bioapplied Chemistry, Faculty of Technology, Osaka City University, Sumiyoshi-ku, 558 Osaka, Japan;(4) Present address: Department of Chemistry, Faculty of Science, Kyoto University, 606 Kyoto, Japan |
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| Abstract: | ![]()
Among the homeotic mutants with altered floral organs, two mutants of Arabidopsis thaliana, apetala3 and pistillata, and two mutants of Antirrhinum majus, deficiens and globosa, have a homeotic conversion of the floral organs in whorl 2 and 3, namely petals to sepals and stamens to carpels. We have isolated a homologue of the DEFICIENS gene from A. thaliana wild type and shown complete complementation of apetala3 mutation by introducing the isolated gene using Agrobacterium-mediated transformation. These results show that the APETALA3 is a homologue of DEFICIENS structurally and functionally. The 5 -upstream region of APETALA3 contains three SRE-like sequence, where MADS box-containing proteins are assumed to bind and regulate expression in tissue-and stage-specific manner. |
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| Keywords: | APETALA3 Arabidopsis thaliana flower homeotic gene flower development MADS box genetic complementation |
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