Effect of different benzyladenine time pulses on the endogenous levels of cytokinins,indole-3-acetic acid and abscisic acid in micropropagated explants of Actinidia deliciosa |
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| Institution: | 1. College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China;2. College of Agronomy, South China Agricultural University, Guangzhou 510642, China;3. Agronomy College of Hunan Agricultural University, Changsha 410000, China;4. Yiyang Agricultural Research Institute, Yiyang 413002, China;1. CETEMAS, Forest and Wood Technology Research Centre, Sustainable Forest Management Area. s/n, 33936, Asturias, Spain;2. SLU (Swedish University of Agricultural Sciences), Umeä, Sweden, Department of Forest Biomaterials and Technology, Skogsmarksgränd, 901 83, Umeä, Sweden;3. E.T.S.I. Montes, Technic University of Madrid, Ciudad Universitaria s/n, 28040, Madrid, Spain;1. Macau Environmental Research Institute, Macau University of Science and Technology, Macau, China;2. School of Environment, Tsinghua University, Beijing, 100084, China;3. College of Civil Engineering, Shenzhen University, Shenzhen, 518060, China;1. Department of Horticultural Science and Biotechnology, Seoul National University, Seoul, 08826, South Korea;2. Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 08826, South Korea;1. National Research Tomsk State University, Lenin Av., 36, 634050 Tomsk, Russia;2. Institute of Experimental Botany, AS CR, Rozvojova 263, 165 02 Prague 6, Czech Republic;3. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Str., 35, 127276 Moscow, Russia;4. Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Kuprevich Str., 5/2, 220141 Minsk, Belarus;5. Kholodny Institute of Botany, National Academy of Sciences of Ukraine, Tereshchenkivska Str., 2, 01601 Kyiv, Ukraine |
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| Abstract: | Actinidia deliciosa apical shoots were cultured in MS liquid medium with different benzyladenine (BA) pulses and using cellulose plugs as support for the explants. Abscisic acid (ABA), indole-3-acetic acid (IAA) and some cytokinins (Cks) zeatin (Z), dihydrozeatin (DHZ), zeatin riboside (ZR), dihydrozeatin riboside (DHZR), N6-isopentenyladenine (iP) and N6-isopentenyladenosine (iPR)] levels were measured in leaves from explants cultured both in the absence and in the presence of BA (4.4 μM) for 30 min, 1, 2 and 35 d. Analyses were carried out after three subcultures at the end of the multiplication phase and after 31 d in ex vitro conditions. A clear relationship between the endogenous content of phytohormones and the growth characteristic of kiwifruit microplants could be confirmed. Microplants with the best growth characteristic were those cultured in presence of BA during 1 d. They were characterised by higher contents of IAA than the others studied, as well as by higher values in the IAA/Cks and IAA/ABA ratios, both at the end of the multiplication phase and after the acclimatisation period. Taken all together, these ratios at the end of the multiplication phase could be used as growth indicators of A. deliciosa explants behaviour under ex vitro conditions. |
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