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| Viili乳制品中干酪乳杆菌的分离鉴定 | |
| 引用本文： | 张书光,张云娟,代卫东,荀绍雄,刘旭川,柴俊,张以芳.Viili乳制品中干酪乳杆菌的分离鉴定[J].微生物学杂志,2012,32(3):47-52. |
| 作者姓名： | 张书光 张云娟 代卫东 荀绍雄 刘旭川 柴俊 张以芳 |
| 作者单位： | 1. 云南农业大学动物科学技术学院,云南昆明,650201 2. 云南农业大学教务处,云南昆明,650201 3. 富源县大河镇农业综合服务中心畜牧办,云南曲靖,655505 |
| 基金项目： | 云南省重点基金，云南省自然科学基金，云南省高端科技人才引进计划项目，云南省高校科技创新团队 |
| 摘 要： | 从引进Viili乳制品中筛选、鉴定出3种优良乳酸菌。采用平板分离法从Viili乳制品中分离3株乳酸菌,通过表型1、6S rRNA的PCR扩增、克隆、测序鉴定。3株乳酸菌的表型鉴定结果符合伯杰细菌鉴定手册中乳杆菌属的干酪乳杆菌,16S rRNA序列同源性分析结果表明3株分离菌与干酪乳杆菌的同源性分别为99.93%、100.00%9、9.78%,从Viili乳制品分离到3株干酪乳杆菌。 |
| 关 键 词： | 干酪乳杆菌 分离 鉴定 16SrRNA |
Isolation and Identification of Lactobacillus casei Strains from Viili Yogurt |
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| ZHANG Shu-guang,ZHANG Yun-juan,DAI Wei-dong,XUN Shao-xiong,LIU Xu-chuan,CHAI Jun,ZHANG Yi-fang.Isolation and Identification of Lactobacillus casei Strains from Viili Yogurt[J].Journal of Microbiology,2012,32(3):47-52. | |
| Authors: | ZHANG Shu-guang ZHANG Yun-juan DAI Wei-dong XUN Shao-xiong LIU Xu-chuan CHAI Jun ZHANG Yi-fang |
| Institution: | 1 (1. Coll. of Anim. Sei. ＆ Teehnol. , 2. Dean＇s Off. , Yunnan Agrie. Uni. , Kunming 650201; 3. Anim. Husb. Off. , Comprehensive Service Ctr. Dahe Town, Fuyuan County＇, Quqing, Yunnan 655505) |
| Abstract: | Three fine lactic acid bacteria strains from imported Viili dairy were screened and isolated adopting plate i- solation method by means of their phenotype, 16S rRNA PCR amplification, cloning, sequencing and other methods. The resuhs showed that their phenotypic identification results were in line with the LactobaciUus casei of the genus de- scribed in Bergey＇s Manual of Determination Bacteriology, 16S rRNA gene sequence homology analysis showed that the homologies of the three isolated strains with LactobaciUus casei were respectively 99.93% , 100.00% , 99.78%. Therefore, all of the three strains isolated from Viili dairy products were Lactobacillus casei. |
| Keywords: | lactic acid bacteria separation identification 16S rRNA |
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