| 一组厌氧真菌菌系产阿魏酸酯酶的初步研究 |
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| 引用本文： | 周皓芹,杨梅玉,佟金,陈玉香.一组厌氧真菌菌系产阿魏酸酯酶的初步研究[J].微生物学杂志,2012,32(4):53-56. |
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| 作者姓名： | 周皓芹 杨梅玉 佟金 陈玉香 |
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| 作者单位： | 吉林大学生物与农业工程学院,吉林长春130025;吉林大学工程仿生教育部重点实验室,吉林长春130022 |
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| 基金项目： | 吉林省科技发展计划项目，吉林大学"985工程"项目 |
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| 摘 要： | 利用玉米秸秆粉为唯一碳源的筛选培养基,从肉牛瘤胃液中筛选构建了一组厌氧真菌菌系,研究了该菌系产阿魏酸酯酶的特征.阿魏酸酯酶的最适pH为8.0,最适温度为40℃,最高酶活力为19.1 mU/mL,在pH 6.0 ～8.0及35～45℃之间,酶活性保持相对稳定.Mg2+对酶活力具有激活作用,Fe2、Cu2+、Fe3+等均抑制酶活力.
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| 关 键 词： | 厌氧真菌 阿魏酸酯酶 产酶特性 |
A Group of Feruloylesterase-Producing Anaerobic Fungal Strains |
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| ZHOU Hao-qin,YANG Mei-yu,TONG Jin,CHEN Yu-xiang.A Group of Feruloylesterase-Producing Anaerobic Fungal Strains[J].Journal of Microbiology,2012,32(4):53-56. |
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| Authors: | ZHOU Hao-qin YANG Mei-yu TONG Jin CHEN Yu-xiang |
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| Institution: | 1,2(1.Coll.of Biol.& Agric.Engin.,Jilin Uni.,Changchun 130025; Key Lab.of Engin.Bionics under Minist.of Educ.,Jilin Uni.,Changchun 130022) |
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| Abstract: | A group of anaerobic fungal strains was screened and established from cattle rumen fluid with screening medium using corn stalk powder as the sole carbon source and studied their feruloylesterase-producing characteristics.The optimum pH of feruloylesterase was at 8.0 with the most suitable temperature at 40 ℃;the highest enzyme activity was 19.1 mU/mL,the enzyme activity remained relatively stable at pH 6.0~8.0 and 35~45 ℃.Mg2+ stimulated the enzyme activity,however,Fe2+,Cu2+,and Fe3+ inhibited the activity. |
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| Keywords: | anaerobic fungi feruloylesterase enzyme-producing characteristics |
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