Vacuolating cytotoxic activity of Pasteurella multocida causing haemorrhagic septicaemia in buffalo and cattle |
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| Authors: | Najmul H Shah Jeike Biewenga Nasir H Shah Frits K de Graaf |
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| Institution: | Botanisches Institut, Universität zu Köln, Gyrhofstr. 15, D-50923 Köln, Germany |
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| Abstract: | Abstract Sequence data had indicated that cyanobacteria might possess a bidirectional hydrogenase with properties similar to the soluble enzymes from Alcaligenes eutrophus, Nocardia opaca and Desulfovibrio fructosovorans . The present study shows that extracts from the cyanobacterium Anacystis nidulans catalyse NAD(P)H-dependent H2 evolution with low but significant activity and uptake of the gas with NAD(P)+ as the electron acceptor. NAD+ is the preferred electron acceptor and NADH the preferred donor compared to NADP+ and NADPH, respectively. Activity levels of this NAD(P)+dependent, bidirectional hydrogenase are too low to support chemoautotrophic growth in A. nidulans . |
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| Keywords: | Bidirectional hydrogenase Hydrogen metabolism Chemoautotrophic growth Cyanobacterium Anacystis nidulans |
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