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Manipulating photosynthesis
Authors:Jacqueline S. Knight  Francisco Madueño  Simon A. Barnes  John C. Gray
Affiliation:(1) School of Biological Sciences, University of Manchester, 3.614 Stopford Building, Oxford Rd., M13 9PT Manchester, UK
Abstract:
The levels of individual photosynthetic proteins can be independently decreased by theAgrobacterium-mediated transformation of plants with antisens RNA constructs. Protocols for the introduction of such constructs intoAgrobacterium, theAgrobacterium-mediated transformation of tobacco leaf disks, and the screening and analysis of the transgenic plants produced are described.
Keywords:Photosynthesis  antisense RNA   Agrobacterium-mediated tobacco transformation
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