湖南汉族人群IL-1O启动子和IL-1ra基因多态性与SLE相关性研究

目的:研究湖南汉族人群IL-10启动子和IL-1受体拮抗剂(IL-1ra)的基因多态性,探讨IL-10启动子和IL-1ra基因多态性与SLE疾病的关系。方法:PCR和限制性内切酶酶切分析SLE患者(n=83)和正常对照人群(n=125)IL-10启动子和IL-1ra基因多态性,对基因频率进行分析。结果:湖南汉族人群IL-1ra及IL-10启动子基因具有多态性;SLE患者IL-1RN*1等位基因的频率显著高于正常对照组(P<0.05,RR=5);SLE患者IL-10启动子区-597位*A、-824位*T和ACC亚型的基因频率高于正常对照组(P<0.001)。结论:SLE患者IL-1RN*1的基因频率、IL-10启动子区-597位和-824位的基因多态性与正常人比较有显著差异,提示以上基因可能与SLE的发病有一定相关性。

Study on the Correlation between IL-10 Promoter,IL-1ra Gene Polymorphism and SLE in Chinese Han Population of Hunan Province

Objective:to study on the gene polymorphisms of interleukin-10(IL-10) promoter and interluekin-1 receptor antagonist(IL-1ra) in the Han nationality of Hunan and test the correlation between these genes and Systemic lupus erythematosus(SLE) patients.Methods:polymerase chain reaction and restriction enzyme cleavage were used to analyze the gene polymorphisms.Results:there are gene polymorphisms in IL-1ra and IL-10 promoter in the Hunan Han groups.IL-1RN*1 frequency of SLE patients increased significantly(P<0.05,RR=5) than that of normal controls.A significant increase was found in the frequencies of-597*A and-824*T and ACC genotype between SLE patients and normal controls (P<0.001).Conclusions:IL-1ra and IL-10 promoter polymorphisms are associated with SLE and may be the genetic predisposition to the disease.