The comparison of immune-enhancing activity of sulfated polysaccharidses from Tremella and Condonpsis pilosula |
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| Authors: | Xiaona Zhao Yuanliang Hu Deyun Wang Jianzhu Liu Liwei Guo |
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| Affiliation: | 1. Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China;2. College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, PR China;3. College of Animal Science, Yangtze University, Jingzhou 434023, PR China |
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| Abstract: | Based on our previous research, four sulfated polysaccharide (sPSs) from Tremella and Condonpsis pilosula, sTPStp, sTPS70c, sCPPStp and sCPPS50c, were prepared and their effects on splenic lymphocytes proliferation in vitro and the immune response of ND vaccine in chicken were compared taking the unmodified polysaccharide (uPS) TPStp as control. The results showed that four sPSs could significantly or numerically stimulate splenic lymphocyte proliferation singly or synergistically with LPS in vitro, sTPS70c and sCPPStp demonstrated better effect; promote peripheral lymphocytes proliferation and enhance serum HI antibody titer in chickens vaccinated with ND vaccine, the actions of sPSs were stronger than that of uPS, and sTPS70c at medium dosage presented the best efficacy. These indicated that sulfation modification could improve the immune-enhancing activity of TPS and CPPS, sTPS70c possessed the strongest activity and would be expected as a component of new-type immunopotentiator. |
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| Keywords: | sPS, sulfated polysaccharide TPS, tremella polysaccharide sTPSs, sulfated tremella polysaccharide sCPPSs, sulfated condonpsis pilosula polysaccharide uPS, unmodified polysaccharide CSA, chlorsulfonic acid Pyr, pyridine LPS, lipopolysaccharide CMF-PBS, calcium and magnesium-free phosphate-buffered saline DS, degree of sulfation MTT, 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide DMSO, dimethyl sulfoxide |
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