Cis effect oflacZ sequences in transgenic mice |
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| Authors: | Andras Paldi Louise Deltour Jacques Jami |
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| Institution: | (1) Agricultural Biotechnology Center, 2101 Gödöllö, P.O. Box 170, Hungary;(2) Institut Cochin de Génétique Moléculaire, CHU Cochin-Port Royal, Unité 257 de l'Institut National de la Santé et de la Recherche Médicale, 24 rue du Faubourg Saint-Jacques, 75014 Paris, France |
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| Abstract: | Transgenic mice carrying the 3-hydroxy-3-methylglutarylCoA reductase (HMG) promoter driving theEscherichia coli  -galactosidase (lacZ) gene did not display the expected ubiquitous and constitutive expression inHMG-lacZ transgenic mice. The same promoter is however able to drive ubiquitous expression of the chloramphenicol acetyltransferase (cat) gene. Two lines of doubleHMG-lacZ andHMG-cat transgenic mice were obtained in which the two constructs were integrated at the same genomic sites. These mice expressed both reporter genes, but exclusively in the testes. These results suggest that thelacZ sequence might interfere negatively with the expression of the adjacentHMG-cat transgene. |
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| Keywords: | HMG-cat gene HMG-lacZ gene embryo testis |
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