Effects of Growth Regulators on Polyamine Content and Peroxidase Activity in Hevea brasiliensis Callus |
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| Authors: | HADRAMI I EL; D'AUZAC J |
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| Institution: | * Laboratory of Plant Physiology, Department of Biology, University of Cadi Ayyad - Faculty of Sciences Semlalia, BP. S15 Marrakech, Morocco
 Laboratory of Applied Plant Physiology, University of Montpellier II F-34095 Montpellier Cedex 5, France |
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| Abstract: | 3, 4-dichlorophenoxyacetic acid (3,4-D) and benzylaminopurine(BAP) at 9 µM (control medium) was compared with 4.5,2.25, and 0.45 µM for ability to induce callogenesis andembryogenesis from seed explants of Hevea brasiliensis. Supplyingthese growth regulators at 4.5 µM for 20 d improved embryogenicpotential compared with the control medium (El Hadrami, Carronand d'Auzac, 1991, Annals of Botany 67, 511–515), sustainedputrescine, spermidine and spermine at a higher level throughoutof much of the culture period (40–70 d), and maintainedlow levels of peroxidase activity. In the control medium, poorcallus embryogenesis is considered a consequence of rapid ageingof tissues characterized by (i) acceleration of an early buttransient production of polyamines, which promoted embryogeniccapacity, and (ii) an early peak in peroxidase activity thatwas positively correlated with callus browning, one of the factorslimiting embryogenesis. Somatic embryogenesis, polyamines, peroxidase, Hevea brasiliensis, rubber-tree |
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