Isolation of infectious xenotropic mouse type C virus by transfection of a heterologous cell with DNA from a transformed mouse cell |
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Authors: | Scolnick E M Bumgarner S J |
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Institution: | Viral Leukemia and Lymphoma Branch, National Cancer Institute, Bethesda, Maryland 20014. |
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Abstract: | An endogenous xenotropic type C virus has been isolated from a Kirsten sarcoma virus-transformed BALB/c mouse cell line by transfection of a mink fibroblast cell with the DNA from the transformed cells. The results indicate that transfection may be used as a technique to isolate this endogenous type C virus without the need to chemically induce the cell line containing the provirus prior to attempting to isolate the virus. |
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