大豆突变基因的遗传分析及窄叶突变基因的ＲＡＰＤ标记

宽叶、无4粒荚大豆品种鲁豆4号（代号LD4）经烷化剂EMS诱变处理选育出窄叶和4粒荚同时发生突变的稳定突变系E182。突变系与亲本杂交遗传分析表明，“宽叶与窄叶”和“无4粒荚与有4粒荚”2对性状均符合孟德尔单因子的31分离比率，说明窄叶和4粒荚突变性状均受单个隐性核基因控制。F

Genetic Analysis of Mutant Genes and RAPD Marker of Mutant Gene of Narrow Leaflet in Soybean

Mutant E182 with “narrow leaflet-4 seeded pod” was selected fromdescendents of EMS-treated seeds of soybean variety Lu Dou No.4 (LD4) with “ovate leaflet-without 4 seeded pod”. Genetic analyses of F2 individuals of crossing between mutant E182 and parent LD4 indicated that segregation ratio between ovate and narrow leaflet was 31, so was segregation ratio between “without 4 seeded pod” and “4 seeded pod”. Segregation ratios of four character types in F2 population of 1 654 individuals were beyond 9331 of two pairs of independent gene, showing linkage inheritance. Reccmbinant ratio between mutant genes of narrow leaflet and 4 seeded pod was 11.24%±0.81%. On the other hand, mutant E182, parent LD4 and F2, F3 individuals of their hybrids were analyzed by means of RAPD technique. The marker OPY6-1300 linked with the mutant gene of narrow leaflet was generated, and genetic distance of the marker and mutant gene was 8 cent Morgan (cM), being 10cM nearer than RFLP marker of narrow leaflet generated by shoemaker.