Selection and Optimization of Culture Medium for Exopolysaccharide Production by Coriolus (Trametes) Versicolor |
| |
| Authors: | A P A Tavares M S M Agapito M A M Coelho J A Lopes da Silva A Barros-Timmons J A J Coutinho A M R B Xavier |
| |
| Institution: | (1) QOPNA; CICECO – Departamento de Química, Universidade de Aveiro, 3810-193 Aveiro, Portugal;(2) Departamento de Engenharia Bioquímica, EQ/UFRJ, Bloco E, Lab.113, 21949-900 Rio de Janeiro, Brasil |
| |
| Abstract: | Summary Coriolus versicolor is a medicinal fungus producing exopolysaccharides (EPS). Five well-defined culture media were studied to select the medium
that maximizes production of EPS by C. versicolor. Biomass, reducing sugars and EPS concentrations along with the rheological behaviour of the broth were followed during fermentations
lasting 9 days. The yeast malt extract medium (YM) was shown to yield the highest production of EPS. Fermentation conditions
with YM medium were further investigated to optimize EPS production by C. versicolor. An experimental design to do this was adopted, in which the effects of pH and initial substrate concentration were considered.
The effects of initial glucose concentration (5, 15 and 25 g l−1) and pH (4.0, 5.5 and 7.0) were evaluated. The initial glucose concentration was found to be the most important factor in
EPS production and also cell growth. |
| |
| Keywords: | Coriolus versicolor exopolysaccharide production experimental design optimization submerged culture |
| 本文献已被 SpringerLink 等数据库收录！ |
|
