R.SspD5I is a neoschizomer of HphI producing blunt end DNA fragments |
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Authors: | Zheleznaya L Shiryaev S Zheleznyakova E Matvienko N Matvienko N |
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Affiliation: | Institute of Theoretical and Applied Biophysics, RAS, Pushchino, Russia. |
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Abstract: | The strain Staphylococcus species D5 produces a restriction enzyme. It is the neoschizomer of HphI endonuclease, which cleaves DNA at a distance of eight nucleotides from the recognition sequences producing blunt end DNA fragments: 5'-GGTGA8N /-3' and 3'-CCACT8N /-5'. |
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