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| 半嵌套式PCR技术区别检测犬细小病毒疫苗株和强毒株的研究 | |
| 引用本文： | 刘忠华 钟翎 黄韧 程树军. 半嵌套式PCR技术区别检测犬细小病毒疫苗株和强毒株的研究[J]. Virologica Sinica, 2003, 18(4): 401-403 |
| 作者姓名： | 刘忠华 钟翎 黄韧 程树军 |
| 作者单位： | [1]广东省实验动物监测所，广东广州，510260 [2]中山大学生命科学院，广东广州，510275 |
| 基金项目： | 第十批广东省重点科技攻关项目(1997-35) |
| 摘 要： | Two pairs of PCR primers were designed according to the sequances of the vaccine strain and virulent strain of CPV. Heminested PCR method was established. Result of the first PCR amplification showed the same amplified products of 574bp length, after the second PCR amplification, the virulent strain produced the length 364bp fragment, but the vaccine strain couldn' t produce that. The products of PCR were examined by electrophoresis and restriction enzyme digestion. The result showed the length of the fragment and enzyme sites were as the same as those designed. The PCR assay of CPV was proved to be specific and sensitive. It shows that this method may be used in discriminating the vaccine strain and virulent strain of CPV or monitoring the vaccinated canine in order to aviod disease and financial losing. |
| 关 键 词： | 半嵌套式PCR技术 检测 犬细小病毒 疫苗株 强毒株 |
Establishment of Polymerase Chain Reaction for Discrimination of Vaccine Strains of Canine parvovirus |
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| LIU Zhong-hua,ZHONG Ling,HUANG Ren,CHENG shu-jun. Establishment of Polymerase Chain Reaction for Discrimination of Vaccine Strains of Canine parvovirus[J]. 中国病毒学(英文版), 2003, 18(4): 401-403 | |
| Authors: | LIU Zhong-hua ZHONG Ling HUANG Ren CHENG shu-jun |
| Affiliation: | LIU Zhong-hua~1,ZHONG Ling~2,HUANG Ren~1,CHENG shu-jun~1 |
| Abstract: | Two pairs of PCR primers were designed according to the sequances of the vaccine strain and virulent strain of CPV. Heminested PCR method was established. Result of the first PCR amplification showed the same amplified products of 574bp length, after the second PCR amplification, the virulent strain produced the length 364bp fragment, but the vaccine strain couldn't produce that. The products of PCR were examined by electrophoresis and restriction enzyme digestion. The result showed the length of the fragment and enzyme sites were as the same as those designed. The PCR assay of CPV was proved to be specific and sensitive. It shows that this method may be used in discriminating the vaccine strain and virulent strain of CPV or monitoring the vaccinated canine in order to aviod disease and financial losing. |
| Keywords: | Parvoviridae Heminested PCR Vaccine strain |
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