| 兔出血症病毒中国株衣壳蛋白基因的克隆和序列分析 |
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| 引用本文： | 严维巍,崔治中,王永坤.兔出血症病毒中国株衣壳蛋白基因的克隆和序列分析[J].Virologica Sinica,2003,18(2):129-133. |
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| 作者姓名： | 严维巍 崔治中 王永坤 |
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| 作者单位： | [1]扬州大学畜牧兽医学院动物医学系，江苏扬州，225009 [2]山东农业大学动物科技系，山东泰安，271018 |
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| 基金项目： | 山东省科学技术发展计划第三批资助项目(2000年387号) |
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| 摘 要： | 应用RT—PCR技术，从兔出血症病毒中国分离株WX84中成功扩增出预期大小为1．7kb的特异性条带，将扩增产物提纯后克隆入pGEM^R—T载体，经转化、筛选及酶切鉴定后，获得了该株病毒衣壳蛋白基因的克隆，序列分析表明扩增的中国株BHD衣壳蛋白基因片段长度为1740bp，共编码580个氨基酸。该核酸序列与其它国家报道的多株BHDV序列相互间同源性高达98．2％一99．0％，其推导的氨基酸序列同源性也达98．3％--99．1％，为极度保守片段。
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| 关 键 词： | 兔 出血症病毒 中国株 衣壳蛋白基因 基因克隆 序列分析 RT－PCR 出血症 |
Cloning and Sequencing of the Capsid Protein Gene of Rabbit hemorrhagic disease virus Isolate WX84 |
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| YAN Wei-wei,CUI Zi-zhong,WANG Yong-kun.Cloning and Sequencing of the Capsid Protein Gene of Rabbit hemorrhagic disease virus Isolate WX84[J].中国病毒学(英文版),2003,18(2):129-133. |
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| Authors: | YAN Wei-wei CUI Zi-zhong WANG Yong-kun |
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| Institution: | YAN Wei-wei1,CUI Zi-zhong2**,WANG Yong-kun1 |
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| Abstract: | The capsid protein (VP60) gene of Rabbit hemorrhagic disease virus (RHDV) isolate WX84 was amplified by RT-PCR and cloned into pGEMR-T vector for sequence. The sequence analysis showed that the nucleotide sequence of the vp60 gene of WX84 was 1740 bp and encoding a protein of 580 amino acids. Sequence comparison with other published RHDV vp60 genes showed that the homology of the nucleotide was between 98.2% and 99.0%. The result showed that vp60 gene was a highly conserved gene. |
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| Keywords: | Rabbit hemorrhagic disease virus Capsid protein gene (vp60) RT-PCR Sequence analysis |
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