Membrane binding of a lipidated N-Ras protein studied in lipid monolayers |
| |
Authors: | Frank Bringezu Monika Majerowicz Shaoying Wen Guido Reuther Kui-Thong Tan Jürgen Kuhlmann Herbert Waldmann Daniel Huster |
| |
Institution: | Institute of Medical Physics and Biophysics, University of Leipzig, Leipzig, Germany. frank.bringezu@medizin.uni-leipzig.de |
| |
Abstract: | The adsorption of doubly lipidated full-length N-Ras protein on 1,2-dipalmitoyl-sn-phosphatidylcholine (DPPC) monolayers was studied by lateral pressure analysis, grazing incidence X-ray diffraction (GIXD),
and specular reflectivity (XR). N-Ras protein adsorbs to the DPPC monolayer (lateral pressure of 20 mN/m) from the subphase
thereby increasing the lateral pressure in the monolayer by 4 mN/m. The protein insertion does not alter the tilt angle and
structure of the lipid molecules at the air/water interface but influences the electron density profile of the monolayer.
Further, electron density differences into the subphase were observed. The Fresnel normalized reflectivity could be reconstructed
in the analysis using box models yielding electron density profiles of the DPPC monolayer in the absence and in the presence
of N-Ras protein. The electron density profiles of the DPPC monolayer in the presence of Ras showed clear intensity variations
in the headgroup/glycerol/upper chain region, the so-called interface region where previous bilayer studies had confirmed
Ras binding.
Dedicated to Prof. K. Arnold on the occasion of his 65th birthday. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|