| 黄嘌呤氧化酶产生菌的离子注入诱变及其二步发酵的研究 |
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| 引用本文： | 易福明,吴显辉,冯胜彦,陈蔚梅.黄嘌呤氧化酶产生菌的离子注入诱变及其二步发酵的研究[J].激光生物学报,2002,11(2):137-141. |
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| 作者姓名： | 易福明 吴显辉 冯胜彦 陈蔚梅 |
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| 作者单位： | 武汉大学生命科学学院,生化与生物物理学系,中国湖北,武汉,430072 |
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| 基金项目： | 85国家重点科技攻关项目 (85 - 0 8- 0 2 - 0 5 )，湖北省科委 95重点科技项目 (95 1716 ) |
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| 摘 要： | 采用剂量 1.0× 10 1 2 ～ 1.0× 10 1 6ions cm2 的N+注入产黄嘌呤氧化酶的球形节杆菌ATCC80 10 ,研究其诱变效果。结果表明 :细菌的存活曲线呈现特殊的“马鞍形” ,菌落形态和颜色发生了变化 ,且变化与产酶量有关 ,经过筛选获得了遗传性能稳定的高酶活突变株 ,使酶活较出发菌提高了 43.0 %。改进发酵工艺 ,采用二步发酵其酶得率是一步发酵的 4.75倍。
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| 关 键 词： | 黄嘌呤氧化酶产生菌 离子注入 诱变 二步发酵 球形节杆菌 |
| 文章编号： | 1007-7146(2002)02-0137-05 |
| 收稿时间： | 2001/9/10 |
| 修稿时间： | 2001年9月10日 |
Ion Implantation Mutation Breeding of Xanthine Oxidase Producing Strain and its Two-step Fermentation Technology |
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| YI Fu-ming,WU Xian-hui,FENG Shen-yan,CHEN Wei-mei.Ion Implantation Mutation Breeding of Xanthine Oxidase Producing Strain and its Two-step Fermentation Technology[J].ACTA Laser Biology Sinica,2002,11(2):137-141. |
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| Authors: | YI Fu-ming WU Xian-hui FENG Shen-yan CHEN Wei-mei |
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| Abstract: | The present paper deals with biological effects of xanthine oxidase producing Arthrobacter globiformis ATCC8010 implanted by nitrogen ions with dose from 1.0×10 12 ～1.0×10 16 ions/cm 2.The results show that the survival of the strain appears "saddle shape",and the colony shapes and colors varied which related to the ability of producing xanthine oxidase. One mutant capable of producing 1.43 times as much xanthine oxidase as its original strain was obtained by selection.The optimization study on fermentation technology for the high-yield strain indicated that the production of xanthine oxidase increased by 4.75 times using two-step fermentation as compared with one-step fermentation. The yield of the xanthine oxidase is 450 units per g xanthine. |
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| Keywords: | ions implantation xanthine oxidase arthrobacter globiformis two-step fermentation |
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