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Isolation of RNA from apple skin
Authors:Deepali Mitra  Arend Kootstra
Institution:(1) The Horticulture and Food Research Institute of NZ Ltd, Private Bag 92 169, Auckland, New Zealand
Abstract:A method has been developed for the isolation of RNA from apple skin. The method involves an adaptation of the Manning (1991) method and includes a high-salt extraction step and a final purification step through a CsCl cushion. The RNA isolated was of high quality and produced good hybridization signals in northern blot analyses.
Keywords:apple skin            Malus spp    polysaccharides  RNA isolation
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