Th1类细胞因子对pHCV-C重组体诱生免疫应答的增强作用

为了探索Th1类细胞因子IL-2和IL-12对含丙型肝炎病毒(HCV)核心(C)基因重组体诱生的免疫应答的增强作用，本文构建了包含HCVC基因片段的重组质粒pHCV-C，将其单独或与Th1类细胞因子表达质粒pIL-2或pIL-12共免疫BALB/c小鼠，ELISA法检测免疫小鼠血清中的HCVC特异性抗体滴度；以pHCV-C转染SP2/0细胞，经筛选稳定表达HCVC抗原者(SP2/0-HCV-C)为靶细胞，

Enhancing Effect of Th1 Cytokine on Immune Responses of Mice to Recombinant Plasmid Encoding Hepatitis C Virus Core Gene

BALB/c mice were immunized with a recombinant plasmid containing HCV-C gene (pcDNAHCV-C) alone or together with a constructs encoding IL-2 or IL-12 (pIL-2 or pIL-12). Anti-HCV-C specific antibody in the sera was measured with ELISA. Spleen cells from experimental and control mice were extracted. The mouse myloma SP2/0 cells expressing HCV C antigen served as target cells. CTL activity was measured by standard 4h 51Cr release assays. The results showed that anti-HCV-C antibody A value increased from 1.21 of pcDNAHCV-C alone to 2.09 and 1.73 of pHCV-C coimmunized with pIL-2 or pIL-12 respectively. CTL activity to HCV core protein was substantially enhanced after coimmunization with the IL-12 expressing plasmid. In contrast, the improvement of HCV core-specific CTL activity with an IL-2-producing construct coimmunization was not obvious, which could related to the low-level expression of IL-2 vector-pDOR. DNA vaccine of HCV combined with IL-12 expressing plasmid could get better immunizing effects, and it might represent a gene therappeutic strategy against the HCV infection of human.