Distribution of beta-N-acetylglucosaminidase, hyaluronoglucosaminidase and acrosin in buffalo and goat spermatozoa.

The distribution of beta-N-acetylglucosaminidase, hyaluronoglucosaminidase and acrosin in buffalo and goat sperm acrosomes was studied. The three hydrolases were found to occur in soluble and bound forms. In the bound form, they were associated with the denuded sperm and were maximally solubilized at pH 3.0. The possible role of beta-N-acetylglucosaminidase in fertilization is discussed.