A scanning electron microscope study of Craspedella sp. from the branchial chamber of redclaw crayfish,Cherax quadricarinatus,from Queensland,Australia |
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Authors: | Kim B. Sewell Lester R. G. Cannon |
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Affiliation: | (1) The Queensland Museum, Cultural Centre, PO Box 3300, 4101 South Brisbane, Queensland, Australia;(2) The Department of Parasitology, The University of Queensland, 4072 Brisbane, Queensland, Australia;(3) The Department of Anatomical Sciences, The University of Queensland, 4072 Brisbane, Queensland, Australia |
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Abstract: | Epidermal topography was examined, including papillate ridges, grooves and ciliated sensory papillae of Craspedella sp. from the branchial chamber of redclaw crayfish, Cherax quadricarinatus, from Queensland, Australia. Rhandites were observed to discharge from ducts opening mainly in a small distal region of the ventral epidermis of the three central (of five) tentacles. These regions, devoid of ciliated sensory papillae, serve to adhere the anterior end of the worms during locomotion. Secretions from glands associated with the posterior attachment organ were observed to discharge from pores on the outside region of the ventral surface of the disc.A comparison of various scanning electron microscopy (SEM) fixation techniques showed that (1) hot fixatives at 90 °C provide most information on the largest number of epidermal structures and (2) different fixation regimes highlight different epidermal features. |
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Keywords: | Temnocephalidae Craspedella ectosymbiotic flatworms scanning electron microscopy epidermis methods of fixation |
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