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db—cAMP对转化细胞钙调素基因表达与细胞骨架的影响
引用本文:柳惠图 王端顺. db—cAMP对转化细胞钙调素基因表达与细胞骨架的影响[J]. 实验生物学报, 1990, 23(3): 273-283
作者姓名:柳惠图 王端顺
摘    要:


关 键 词:db-cAMP 细胞骨架 CaM基因表达

The effect of db-cAMP on the gene expression of calmodulin and cytoskeleton in the transformed cells
H T Liu,D S Wang,H Q Zhang,S B Xue,J S You,C Y Du,X L Wang. The effect of db-cAMP on the gene expression of calmodulin and cytoskeleton in the transformed cells[J]. Acta Biologiae Experimentalis Sinica, 1990, 23(3): 273-283
Authors:H T Liu  D S Wang  H Q Zhang  S B Xue  J S You  C Y Du  X L Wang
Affiliation:Department of Biology, Beijing Normal University, China.
Abstract:
We have demonstrated that the distribution of microtubules (MT), microfilaments (MF) and fibronectin (FN) were diminished, while the gene expression of the calmodulin and c-fos enhanced in the transformed C3 H10 T1/2 cells. After treatment with 1 mM db-cAMP for 1 hr. and 2 hrs., there was an early and rapidly reduced in gene expression of calmodulin and c-fos respectively. After db-cAMP treatment for 4-5 days, the number of Capping cells of ConA binding decreased significantly and the cell surface microvilli decreased also. The growth of treated cells was inhibited markedly. By using 4F1 cDNA probe, which is preferentially expressed in G1 phase, we have found that the db-cAMP treated cells were accumulated at G1 phase. Of particular interest is the fact that the distribution of microtubules, microfilaments and fibronectin were recovered after treatment with 1 mM db-cAMP for 6 days. It is suggested that the inhibition of proliferation, alteration of phenotype and recovery of cytoskeleton in transformed cells after treatment with db-cAMP are related to the inhibition of gene expression of calmodulin.
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