高盐溶液预处理幽门螺杆菌对蒙古沙土鼠胃黏膜损伤作用

目的 探讨高盐预处理的幽门螺杆菌(H.pylori)对胃黏膜的损伤作用。 方法 将30%高盐预处理前后的胃癌来源的H.pylori菌株(4854)灌胃蒙古沙土鼠(MGs),在灌胃后13、26和73周解剖动物,通过组织病理学检查、免疫组化染色和黏膜厚度测量,探讨高盐预处理的H.pylori对胃黏膜的损伤作用。 结果 与未加盐预处理的相应菌株相比,高盐预处理组小鼠的慢性炎症、黏膜变性/坏死、腺体萎缩伴肠上皮化生的发生率较低,黏膜糜烂/溃疡和黏膜上皮增生的发生率较高,差异均有统计学意义(t=8.325 6,P=0.040 8)。第73周,高盐预处理4854菌株组胃体和胃窦黏膜增生显著高于未加盐预处理组(t=12.802 4,P=0.035 1；t=16.536 0,P=0.043 8)。 结论 高盐预处理改变了H.pylori的体内致病性,有助于阐明H.pylori感染与高盐饮食在胃病中的相互作用模式。

Effects of Helicobacter pylori pretreated with 30% NaCl solution on gastric mucosal injury in Mongolian gerbils

Objective To observe the damaging effect of H.pylori pretreated with high salt solution on gastric mucosa. Methods Mongolian gerbils (MGs) were administered intragastrically gastric cancer derived H.pylori strains (4854) with and without pretreatment of 30% high salt solution, respectively. The animals were dissected at 13,26 and 73 weeks after the intragastrically administration. By using histopathology, immunohistochemical staining and mucosal thickness measurement, the salt pretreated H.pylori induced gastric mucosa injury was observed. Results The incidence of chronic inflammation, mucosal degeneration/necrosis, gland atrophy and intestinal metaplasia was lower, while that of mucosal erosion/ulcer and epithelial hyperplasia was significantly higher in the high salt pretreatment group than that in the control group, respectively (t=8.325 6, P=0.040 8). At the 73rd week, the proliferation of gastric body and antrum mucosa in the salt pretreated group was significantly higher than that in the control group (t=12.802 4, P=0.035 1; t=16.536 0, P=0.043 8). Conclusion High salt pretreatment changed the pathogenicity of H.pylori in vivo, which is helpful to clarify the interaction between H.pylori infection and high salt diet in gastric diseases.