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The cortical actin cytoskeleton in a Dipteran embryo: Analysis of the spatial reorganization of F-actin aggregates during the early nuclear division cycles
Authors:Maria Giovanna Riparbelli  Romano Dallai  Giuliano Callaini
Abstract:Rhodamine phalloidin-staining was used to study the organization of the cortical actin cytoskeleton of the early Ceratitis capitata embryo. The dynamics of the actin aggregates and their changes in distribution during the formation of the syncytial blastoderm, were followed in detail. It was found that these aggregates formed a shell-like cluster around the interphase nuclei, and concentrated toward the poles of the mitotic apparatus when the nuclei divided. Laser scanning confocal microscopy revealed that aggregates not clustered at the poles of the mitotic apparatus were closely associated with fine fibers of a dense cytoplasmic network of actin filaments.
Keywords:mitosis  actin localization  Ceralitis embryo
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