The Assembly Domain of the Small Capsid Protein of Kaposi's Sarcoma-Associated Herpesvirus |
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| Authors: | Dale Kreitler Christopher M Capuano Brandon W Henson Erin N Pryce Daniel Anacker J Michael McCaffery Prashant J Desai |
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| Institution: | aViral Oncology Program, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins;bIntegrated Imaging Center, Department of Biology, Johns Hopkins University, Baltimore, Maryland, USA |
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| Abstract: | Self-assembly of Kaposi''s sarcoma-associated herpesvirus capsids occurs when six proteins are coexpressed in insect cells using recombinant baculoviruses; however, if the small capsid protein (SCP) is omitted from the coinfection, assembly does not occur. Herein we delineate and identify precisely the assembly domain and the residues of SCP required for assembly. Hence, six residues, R14, D18, V25, R46, G66, and R70 in the assembly domain, when changed to alanine, completely abolish or reduce capsid assembly. |
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