Effects of sucrose concentration,supporting material and number of air exchanges of the vessel on the growth of in vitro coffee plantlets |
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Authors: | Nguyen Quynh Thi Kozai Toyoki Van Nguyen Uyen |
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Institution: | (1) Laboratory of Environmental Control Engineering, Faculty of Horticulture, Chiba University, Matsudo 648, Chiba 271-8510, Japan;(2) Laboratory of Plant Cell Technology, Institute of Tropical Biology, NCST-VN, 1 Mac Dinh Chi Street, Ho Chi Minh City, Vietnam |
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Abstract: | Growth of coffee (Coffea arabusta) plantlets cultured in vitroas affected by sugar, types of supporting material and number of air exchanges of the vessel was investigated. Single node
cuttings of in vitro coffee plantlets were cultured on half strength MS medium with or without 20 g l−1 sucrose. Two types of supporting material, agar and Florialite, and two levels of air exchange expressed by number of air
exchanges per vessel, 0.2 and 2.3 h−1, were studied. At the end of a 40-day culture period, fresh weight, shoot length, root length and leaf area of plantlets
when cultured on Florialite soaked in sugar-free medium and under the higher number of air exchanges were greater than those
in sugar containing medium. Callus was observed at the shoot base of plantlets grown on agar medium containing sucrose. Photosynthetic
ability of coffee plantlets in vitro was also significantly increased when grown on sugar-free medium with the high number of air exchanges and Florialite as
a supporting material.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | CO2 compensation point Coffea arabusta dissolved O2 diffusivity Florialite hyperhydricity rubisco |
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