Characterization and generation of Escherichia coli adenylate cyclase deletion mutants. |
| |
| Authors: | S Shah and A Peterkofsky |
| |
| Affiliation: | Laboratory of Biochemical Genetics, National Heart, Lung, and Blood Institute, Bethesda, Maryland 20892. |
| |
| Abstract: | ![]()
Escherichia coli delta cya-283 is a 75-bp in-frame deletion overlapping the 5' end of delta cya-854; delta cya-201 is a 41-bp frameshift deletion overlapping the 3' end of delta cya-854. Sequence repeats were found at the boundaries of delta cya-283 and delta cya-201, suggesting a mechanism for deletion formation. Recombinant DNA procedures were used to construct a strain in which the total cya structural gene in the chromosome was replaced by the kanamycin resistance gene. |
| |
| Keywords: | |
|
|
