SSR Markers Closely Linked to the Pi-z Locus are Useful for Selection of Blast Resistance in a Broad Array of Rice Germplasm |
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Authors: | Robert Fjellstrom Anna M McClung A Robert Shank |
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Institution: | (1) United States Department of Agriculture, Agricultural Research Service, Rice Research Unit Beaumont, TX 77713, USA |
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Abstract: | Pi-z is a disease resistance gene that has been effectively used to combat a broad-spectrum of races of the rice blast fungus
Magnaporthe grisea. Although DNA markers have been reported for selection of the Pi2(t) and Pi-z resistance genes at the Pi-z locus, markers that are more tightly linked to the Pi-z locus would benefit rapid and effective cultivar development. Analysis of the publicly available genome sequence of Nipponbare
near the Pi-z locus revealed numerous SSRs that could be converted into markers. Three SSRs on rice PAC AP005659 were found to be very
tightly linked to the Pi-z locus, with one marker, AP5659-3, co-segregating with the Pi-z resistance reaction. The Pi-z factor conferring resistance to two races of blast was mapped to a 57 kb region on the physical map of Nipponbare in a location
where the Pi2(t) gene was physically mapped. Two SSR marker haplotypes were unique for cultivars carrying the Pi-z gene, which indicates these markers are useful for selection of resistance genes at the Pi-z locus in rice germplasm. |
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Keywords: | Magnaporthe grisea Marker assisted selection Microsatellite Oryza sativa |
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