Generation of mutants of a strain producing the new antibiotic batumin by means of transposon mutagenesis |
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| Authors: | V V Smirnov L N Churkina A N Kravets V I Perepnikhatka |
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| Institution: | (1) Zabolotnyi Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, ul. Zabolotnogo 154, 252143 Kiev, Ukraine;(2) Institute of Epidemiology and Infectious Diseases, Ministry of Public Health of Ukraine, 252038 Kiev, Ukraine |
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| Abstract: | Various plasmids carrying transposon Tn5 were used to generate insertion mutants synthesizing batumin, a unique antibiotic
with a selective antistaphylococcal effect. One of the plasmids used provided a sufficient yield of the clones in question.
An analysis of over 7000 clones allowed us to select the mutant clones with increased and decreased levels of batumin synthesis
and the mutants that lost the ability to synthesize this antibiotic. |
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