Detection of gene flow from GM to non-GM watermelon in a field trial |
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Authors: | Chang-Gi Kim Bumkyu Lee Dae In Kim Ji Eun Park Hyo-Jeong Kim Kee Woong Park Hoonbok Yi Soon-Chun Jeong Won Kee Yoon Chee Hark Harn Hwan Mook Kim |
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Institution: | (1) Bio-Evaluation Center, KRIBB, 363-883 Cheongwon, Korea;(2) Biotechnology Institute, Nongwoo Bio Co., Ltd., 469-885 Yeoju, Korea |
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Abstract: | Gene flow from genetically modified (GM) crops to conventional non-GM crops is a serious concern for protection of conventional
and organic farming. Gene flow from GM watermelon developed for rootstock use, containing cucumber green mottle mosaic virus
(CGMMV)-coat protein (CP) gene, to a non-GM isogenic control variety “Clhalteok” and grafted watermelon “Keumcheon” was investigated
in a small scale field trial as a pilot study. Hybrids between GM and non-GM watermelons were screened from 1304 “Chalteok”
seeds and 856 “Keumcheon” seeds using the duplex PCR method targeting theCGMMV- CP gene as a marker. Hybrids were found in all pollen recipient plots. The gene flow frequencies were greater for “Chaiteok”
than for “KeumcheonD; with 75% outcrossing in the “Chaiteok” plot at the closest distance (0.8 m) to the GM plot. A much larger
scale field trial is necessary to identify the isolation distance between GM and non-GM watermelon, as the behaviors of insect
pollinators needs to be clarified in Korea. |
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Keywords: | Citrullus lanatus gene flow genetically modified crop watermelon |
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