| 大鼠脑缺血再灌注后Cathepsin D和caspase-9表达的动态变化 |
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| 引用本文： | 张智博,刘柳青,龚晓燕,彭旭,陈本阳.大鼠脑缺血再灌注后Cathepsin D和caspase-9表达的动态变化[J].现代生物医学进展,2010,10(5). |
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| 作者姓名： | 张智博 刘柳青 龚晓燕 彭旭 陈本阳 |
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| 作者单位： | 1. 长沙市第一医院神经内科,湖南,长沙,410005
2. 南华大学,湖南,衡阳,421001 |
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| 摘 要： | 目的:探讨大鼠缺血再灌注后溶酶体组织蛋白酶D(Cathepsin D CD)、caspase-9在不同时段蛋白质及mRNA表达变化。方法:将60只S D大鼠随机分为三组:正常组(10只),假手术组(10只),脑缺血再灌注组(40只),线栓法制备大脑中动脉梗死模型(MCAO),免疫组化及RT-PCR法分别检测Cathepsin D、caspase-9的蛋白和mRNA表达。结果:与正常组和假手术组比较,模型组大鼠脑缺血再灌注损伤后6h Cathepsin D的蛋白和mRNA表达明显增强(P<0.05),24h达高峰,48h仍保存高水平。caspase-9蛋白和mRNA6h开始明显升高,12h达高峰,此后缓慢下降,但48h组仍显著高于对照组(P<0.05),结论:Cathepsin D、caspase-9在大鼠脑缺血再灌注后表达增强,溶酶体可能参与了脑缺血再灌注损伤后神经细胞凋亡的过程。
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| 关 键 词： | 脑缺血再灌注 溶酶体 CathepsinD caspase-9 |
Changes of CathepsinD and caspase-9 Expression in Rats after Cerebral Ischemia-Reperfusion |
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| ZHANG Zhi-bo,LIU liu-qing,GONG Xiao-yan,PENG xu,CHEN Ben-yang.Changes of CathepsinD and caspase-9 Expression in Rats after Cerebral Ischemia-Reperfusion[J].Progress in Modern Biomedicine,2010,10(5). |
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| Authors: | ZHANG Zhi-bo LIU liu-qing GONG Xiao-yan PENG xu CHEN Ben-yang |
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| Institution: | ZHANG Zhi-bo1,LIU liu-qing2,GONG Xiao-yan2,PENG xu1,CHEN Ben-yang2 1 Department of Neurology,The First Hospitol of Changsha,Changsha 410005,China 2 Nanhua Universit,hengyang,421001,China |
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| Abstract: | Objective:To study the changes of the protein and mRNA of Cathepsin D and caspase-9 expression in different time point in rat after cerebral ischemia-reperfusion.Methods:60 SD rats were randomly divided into three groups: normal group (n=10),sham-operation group (n=10),cerebral ischemia-reperfusion group (n=40),prepared by suture middle cerebral artery infarction model (MCAO),the expression of the protein and mRNA of Cathepsin D and caspase-9were respectively evaluated by methods of immunohistochemistry and... |
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| Keywords: | cerebral ischemia-reperfusion lysosome CathepsinD caspase-9 |
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