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Construction and validation of a GFP-based vector for promoter expression analysis in the fish pathogen Flavobacterium psychrophilum |
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| Authors: | Gómez Esther Pérez-Pascual David Fernández Lucía Méndez Jessica Reimundo Pilar Navais Roberto Guijarro José A |
| Institution: | Departamento de Biología Funcional, Universidad de Oviedo, Oviedo, Asturias, Spain. |
| Abstract: | |
| Keywords: | A525 absorvance at 525 nm ANOVA analysis of variance cfu colony-forming unit DNA deoxyribonucleic acid FRU fluorescence relative unit GFP green fluorescent protein MCS multiple cloning site NA nutrient agar NAC nutrient agar charcoal NB nutient broth PCR polymerase chain reaction ORF open reading frame PBS phosphate buffered saline rpm revolutions per minute |
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